Journal: bioRxiv
Article Title: Tailoring a glycerol-free cryopreservation protocol with anti-freeze (glycol)proteins for commercial and native breeds of chicken
doi: 10.1101/2025.07.18.664799
Figure Lengend Snippet: Identification of very early (oviductal) embryo death. Germinal discs were gently removed from the vitelline membrane, immersed in 0.96 % NaCl and stained on microscope slides with propidium iodide. The presence of cell nuclei is an indicator of fertility. Unfertile germinal disc (left) and fertile germinal disc (right). (Photographs taken by Dr. Krisztina Liptói, NBGK)
Article Snippet: The slides where mounted and analysed under a phase contrast microscope (Zeiss Axioskop 2 plus, camera Micro Publisher 6, Canada) at 1000× magnification.
Techniques: Membrane, Staining, Microscopy